This picture showsRalf Takors

Prof. Dr.-Ing.

Ralf Takors



+49 711 685-64535
0711 685-55164

Business card (VCF)

Allmandring 31
70569 Stuttgart
Room: 1.305

Office Hours

On demand, arrangement via secretary (Ms. Reu).


My key research interest is to understand metabolism and regulation of microbial and mammalian cells in depth, thereby enabling model-based strain and process engineering finally to transfer novel bioprocesses from lab to industrial scale. As such, research activities are rooted in modern biochemical engineering closely following the mindset of systems metabolic engineering and also using tools of synthetic biology.

Peer Reviewed Journal Publications:
  1. 2020

    1. 110. Müller,F., Rapp,J., Hacker,A.-L., Feith,A., Takors,R. and Blombach,B. (2020) CO2/HCO3- accelerates iron reduction through phenolic compounds. mbio.
    2. 109. Verhagen,N., Wijaya,A.W., Teleki,A., Fadhlullah,M., Unsöld,A., Schilling,M., Heinrich,C. and Takors,R. (2020) Comparison of l-tyrosine containing dipeptides reveals maximum ATP availability for l-prolyl-l-tyrosine in CHO cells. Engineering in Life Science, 10.1002/elsc.202000017.
    3. 108. Hermann,M., Teleki,A., Weitz,S., Niess,A., Freund,A., Bengelsdorf,F.R. and Takors,R. (2020) Electron availability in CO2, CO and H2 mixtures constrains flux distribution, energy management and product formation in Clostridium ljungdahlii. Microbial Biotechnology, 10.1111/1751-7915.13625.
    4. 107. Ankenbauer,A., Schäfer,R., Viegas,S.C., Pobre,V., Voß,B., Arraiano,C.M. and Takors,R. (2020) Pseudomonas putida KT2440 is naturally endowed to withstand industrial-scale stress conditions. micobial biotechnology, 10.1111/1751-7915.13571.
    5. 106. Verhagen,N., Teleki,A., Heinrich,C., Schilling,M., Unsoeld,A. and Takors,R. (2020) S-adenosylmethionine and methylthioadenosine boost cellular productivities of antibody forming Chinese hamster ovary cells. Biotechnol. Bioeng., 10.1002/bit.27484.
    6. 105. Kuschel,M. and Takors,R. (2020) Simulated oxygen and glucose gradients as a prerequisite for predicting industrial scale performance a priori. Biotechnol. Bioeng., 10.1002/bit.27457.
    7. 104. Siebler,F., Lapin,A. and Takors,R. (2020) Synergistically Applying 1D modeling and CFD for Designing Industrial Scale Bubble Column Syngas Bioreactors. Engineering in Life Science, 10.1002/elsc.201900132.
  2. 2019

    1. 103. Takors,R. (2019) Biochemical engineering provides mindset, tools and solutions for the driving questions of a sustainable future. Engineering in Life Science, 10.1002/elsc.201900150.
    2. 102. Graf,M., Haas,T., Müller,F., Buchmann,A., Harm-Betbenbetova,J., Freund,A., Nieß,A., Persicke,M., Kalinowski,J., Blombach,B., et al. (2019) Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate. Frontiers in Microbiology, 10.3389/fmicb.2019.01648.
    3. 101. Nitschel,R., Ankenbauer,A., Welsch,I., Wirth,N.T., Massner,C., Ahmad,N., McColm,S., Borges,F., Fotheringham,I., Takors,R., et al. (2019) Engineering Pseudomonas putida KT2440 for the production of isobutanol. Engineering in Life Science, 20, 148–159.
    4. 100. Grenz,S., Baumann,P.T., Rückert,C., Nebel,B.A., Siebert,D., Schwentner,A., Eikmanns,B.J., Hauer,B., Kalinowsk, i J., Takors,R., et al. (2019) Exploting Hydrogenophage pseudoflava for aerobic syngas-based production of chemicals. Metabolic Engineering, 55, 220–230.
    5. 99. Junghans,L., Teleki,A., Wijaya,A., Becker,M., Schweikert,M. and Takors,R. (2019) From nutrional wealth to autophagy: In vivo metabolic dynamics in the cytosol, mitochondrion and shuttles of IgG producing CHO cells. Metabolic Engineering, 10.1016/j.ymben.2019.02.005.
    6. 98. Straathof,A.J.J., Wahl,S.A., Benjamin,K.R., Takors,R., Wierckx,N. and Noorman,H.J. (2019) Grand Research Challenges for Sustainable Industrial Biotechnology. Trends in Biotechnology,
    7. 97. Feith,A., Teleki,A., Graf,M., Favilli,L. and Takors,R. (2019) HILIC-Enabled 13C Metabolomics Strategies: Comparing Quantitative Precision and Spectral Accuracy of QToF High - and QQQ Low-Resolution Mass Spectrometry. Metabolites, 10.3390/metabo9040063.
    8. 96. Haas,T., Graf,M., Nieß,A., Busche,T., Kalinowski,J., Blombach,B. and Takors,T. (2019) Identifying the Growth Modulon of Corynebacterium glutamicum. Frontiers in Microbiology, 10.
    9. 95. Ziegler,M. and Takors,R. (2019) Minimal Cells: Design, Construction, Biotechnological Applications. In Lara,A.R., Gosset Lagarda,G. (eds).
    10. 94. Schwentner,A., Feith,A., Münch,E., Stiefelmaier,J., Lauer,I., Favilli,L., Massner,C., Öhrlein,J., Grund,B., Hüser,A., et al. (2019) Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum. Biotechnology for Biofuels, 12.
    11. 93. Becker,M., Junghans,L., Teleki,A., Bechmann,J. and Takors,R. (2019) Perfusion cultures equire optimum respiratory ATP supply to maximize cell‐specific and volumetric productivities. Biotechnol. Bioeng., 10.1002/bit.26926.
    12. 92. Pobre,V., Graça-Lopes,G., Saramago,M., Ankenbauer,A., Takors,T., Arraiano,C.M. and Viegas,S.C. (2019) Prediction of novel non-coding RNAs relevant for the growth of Pseudomonas putida in a bioreactor. Microbiology.
    13. 91. Nieß,A., Siemann-Herzberg,M. and Takors,R. (2019) Protein production in Escherichia coli is guided by the trade‑off between intracellular substrate availability and energy cost. Microbial Cell Factories, 18.
    14. 90. Siebler,F., Lapin,A., Hermann,M. and Takors,R. (2019) The impact of CO gradients on C. ljungdahlii in a 125 m3 bubble column: Mass transfer, circulation time and lifeline analysis. Chemical Engineering Science, 207, 410–423.
    15. 89. Becker,M., Junghans,L., Teleki,A., Bechmann,J. and Takors,R. (2019) The less the better: How suppressed base addition boosts production of monoclonal antibodies with Chinese Hamster Ovary Cells. Frontiers in Bioengineering and Biotechnlogy.
  3. 2018

    1. 88. Barbosa,S., Niebel,B., Wolf,S., Mauch,K. and Takors,R. (2018) A guide to gene regulatory network interference for obtaining predictive solutions: Underlying assumptions and fundamental biological and data constraints. BioSystems, 174, 37–48.
    2. 87. Lange,J., Münch,E., Müller,J., Busche,T., Kalinowski,J., Takors,R. and Blombach,B. (2018) Deciphering the Adaptation of Corynebacterium glutamicum in Transition from Aerobiosis via Microaerobiosis to Anaerobiosis. Genes, 9, 297.
    3. 86. Lange,J., Mueller,F., Takors,R. and Blombach,B. (2018) Harnessing novel chromosomal integration loci to utilize an    organosolv-derived hemicellulose fraction forisobutanol production with    engineered Corynebacterium glutamicum. MICROBIAL BIOTECHNOLOGY, 11, 257–263.
    4. 85. Zieringer,J. and Takors,R. (2018) In Silico Prediction of Large-Scale Microbial Production Performance: Constraints for Getting Proper Data-Driven Models. Computational and Structural Biotechnology Journal, 16, 246–256.
    5. 84. Schwentner,A., Feith,A., Münch,E., Busche,T., Rückert,C., Kalinowskis,J., Takors,R. and Blombach,B. (2018) Metabolic engineering to guide evolution - Creating a novel mode for L-valine production with Corynebacterium glutamicum. Metabolic Engineering, 47, 31–41.
    6. 1. Graf,M., Zieringer,J., Haas,T., Nieß,A., Blombach,B. and Takors,R. (2018) Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions. Frontiers in Microbiology, 9.
    7. 83. Takors,R., Kopf,M., Mampel,J., Bluemke,W., Blombach,B., Eikmanns,B., Bengelsdorf,F.R., Weuster‐Botz,D. and Dürre,P. (2018) Using gas mixtures of CO, CO2 and H2 as microbial substrates: the do’s and don’ts of successful technology transfer from laboratory to production scale. Microbial Biotechnology, 11.
  4. 2017

    1. 82. Wolf,S., Barbosa,S., Bücher,J. and Takors,R. (2017) Automatic network generation describes differential gene data in user friendly and expeditiously analyzable network views. 2, 1–14.
    2. 81. Delvigne,F., Takors,R., Mudde,R., van Gulik,W. and Noorman,H. (2017) Bioprocess scale-up/down as integrative enabling technology: from fluid    mechanics to systems biology and beyond. MICROBIAL BIOTECHNOLOGY, 10, 1267–1274.
    3. 80. Eigenstetter,G. and Takors,R. (2017) Dynamic modeling reveals a three-step response of Saccharomyces    cerevisiae to high CO2 levels accompanied by increasing ATP demands. FEMS YEAST RESEARCH, 17.
    4. 79. Michalowski,A., Siemann-Herzberg,M. and Takors,R. (2017) Escherichia coli HGT: Engineered for high glucose throughput even under    slowly growing or resting conditions. METABOLIC ENGINEERING, 40, 93–103.
    5. 78. Niess,A., Failmezger,J., Kuschel,M., Siemann-Herzberg,M. and Takors,R. (2017) Experimentally Validated Model Enables Debottlenecking of in Vitro    Protein Synthesis and Identifies a Control Shift under in Vivo    Conditions. ACS SYNTHETIC BIOLOGY, 6, 1913–1921.
    6. 77. Hoffart,E., Grenz,S., Lange,J., Nitschel,R., Mueller,F., Schwentner,A., Feith,A., Lenfers-Luecker,M., Takors,R. and Blombach,B. (2017) High Substrate Uptake Rates Empower Vibrio natriegens as Production Host    for Industrial Biotechnology. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83.
    7. 76. Kuschel,M., Siebler,F. and Takors,R. (2017) Lagrangian Trajectories to Predict the Formation of Population Heterogeneity in Large-Scale Bioreactors. Bioengineering, 4, 27.
    8. 75. Niess,A., Loeffler,M., Simen,J.D. and Takors,R. (2017) Repetitive Short-Term Stimuli Imposed in Poor Mixing Zones Induce    Long-Term Adaptation of E-coli Cultures in Large-Scale Bioreactors:    Experimental Evidence and Mathematical Model. FRONTIERS IN MICROBIOLOGY, 8.
    9. 74. Hellmers,F., Takors,R. and Thum,O. (2017) Robust Enzyme Immobilizates for Industrial Isomalt Production. Molecular Catalysis, 445, 293–298.
    10. 73. Teleki,A., Rahnert,M., Bungart,O., Gann,B., Ochrombel,I. and Takors,R. (2017) Robust identification of metabolic control for microbial L-methionine    production following an easy-to-use puristic approach. METABOLIC ENGINEERING, 41, 159–172.
    11. 72. Loeffler,M., Simen,J.D., Mueller,J., Jaeger,G., Laghrami,S., Schaeferhoff,K., Freund,A., Takors,R. and RecogNice-Team (2017) Switching between nitrogen and glucose limitation: Unraveling    transcriptional dynamics in Escherichia coli. JOURNAL OF BIOTECHNOLOGY, 258, 2–12.
    12. 71. Simen,J.D., Loeffler,M., Jaeger,G., Schaeferhoff,K., Freund,A., Matthes,J., Mueller,J., Takors,R. and RecogNice-Team (2017) Transcriptional response of Escherichia coli to ammonia and glucose    fluctuations. MICROBIAL BIOTECHNOLOGY, 10, 858–872.
    13. 70. Lange,J., Mueller,F., Bernecker,K., Dahmen,N., Takors,R. and Blombach,B. (2017) Valorization of pyrolysis water: a biorefinery side stream, for    1,2-propanediol production with engineered Corynebacterium glutamicum. BIOTECHNOLOGY FOR BIOFUELS, 10.
    14. 69. Lange,J., Takors,R. and Blombach,B. (2017) Zero-growth bioprocesses: A challenge for microbial production strains    and bioprocess engineering. ENGINEERING IN LIFE SCIENCES, 17, 27–35.
  5. 2016

    1. 68. Sudarsan,S., Blank,L.M., Dietrich,A., Vielhauer,O., Takors,R., Schmid,A. and Reuss,M. (2016) Dynamics of benzoate metabolism in Pseudomonas putida KT2440. Metabolic Engineering Communications, 3, 97–110.
    2. 66. Takors,R. and de Lorenzo,V. (2016) Editorial overview: Microbial systems biology: systems biology prepares    the ground for successful synthetic biology. CURRENT OPINION IN MICROBIOLOGY, 33, VIII–X.
    3. 67. Takors,R. (2016) Editorial: How can we ensure the successful transfer from lab- to    large-scale production? ENGINEERING IN LIFE SCIENCES, 16, 587.
    4. 65. Loeffler,M., Simen,J.D., Jaeger,G., Schaeferhoff,K., Freund,A. and Takors,R. (2016) Engineering E-coli for large-scale production - Strategies considering    ATP expenses and transcriptional responses. METABOLIC ENGINEERING, 38, 73–85.
    5. 64. Lieder,S., Jahn,M., Koepff,J., Mueller,S. and Takors,R. (2016) Environmental stress speeds up DNA replication in Pseudomonas putida in    chemostat cultivations. BIOTECHNOLOGY JOURNAL, 11, 155–163.
    6. 63. Pfizenmaier,J., Junghans,L., Teleki,A. and Takors,R. (2016) Hyperosmotic stimulus study discloses benefits in ATP supply and reveals    miRNA/mRNA targets to improve recombinant protein production of CHO    cells. BIOTECHNOLOGY JOURNAL, 11, 1037–1047.
    7. 62. Junghans,L., Teleki,A., Becker,M. and Takors,R. (2016) LC-MS-based compartment-specific metabolome analysis in CHO. NEW BIOTECHNOLOGY, 33, S33–S34.
    8. 61. Wutz,J., Lapin,A., Siebler,F., Schaefer,J.E., Wucherpfennig,T., Berger,M. and Takors,R. (2016) Predictability of k(L)a in stirred tank reactors under multiple    operating conditions using an Euler-Lagrange approach. ENGINEERING IN LIFE SCIENCES, 16, 633–642.
    9. 60. Sanchez-Kopper,A., Becker,M., Pfizenmaier,J., Kessler,C., Karau,A. and Takors,R. (2016) Tracking dipeptides at work-uptake and intracellular fate in CHO culture. AMB EXPRESS, 6.
  6. 2015

    1. 59. Teleki,A., Sanchez-Kopper,A. and Takors,R. (2015) Alkaline conditions in hydrophilic interaction liquid chromatography for    intracellular metabolite quantification using tandem mass spectrometry. ANALYTICAL BIOCHEMISTRY, 475, 4–13.
    2. 58. Vallon,T., Simon,O., Rendgen-Heugle,B., Frana,S., Mueckschel,B., Broicher,A., Siemann-Herzberg,M., Pfannenstiel,J., Hauer,B., Huber,A., et al. (2015) Applying systems biology tools to study n-butanol degradation in    Pseudomonas putida KT2440. ENGINEERING IN LIFE SCIENCES, 15, 760–771.
    3. 57. Pfizenmaier,J., Matuszczyk,J.-C. and Takors,R. (2015) Changes in intracellular ATP-content of CHO cells as response to    hyperosmolality. BIOTECHNOLOGY PROGRESS, 31, 1212–1216.
    4. 56. Blombach,B. and Takors,R. (2015) CO2 - Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Production Processes. Front Bioeng Biotechnol, 3, 108.
    5. 55. Matuszczyk,J.-C., Teleki,A., Pfizenmaier,J. and Takors,R. (2015) Compartment-specific metabolomics for CHO reveals that ATP pools in    mitochondria are much lower than in cytosol. BIOTECHNOLOGY JOURNAL, 10, 1639–1650.
    6. 54. Lieder,S., Nikel,P.I., de Lorenzo,V. and Takors,R. (2015) Genome reduction boosts heterologous gene expression in Pseudomonas    putida. MICROBIAL CELL FACTORIES, 14.
    7. 53. Rimbon,J., Sanchez-Kopper,A., Wahl,A. and Takors,R. (2015) Monitoring intracellular protein degradation in antibody-producing    Chinese hamster ovary cells. ENGINEERING IN LIFE SCIENCES, 15, 499–508.
  7. 2014

    1. 52. Buchholz,J., Graf,M., Freund,A., Busche,T., Kalinowski,J., Blombach,B. and Takors,R. (2014) CO2/HCO3 (-) perturbations of simulated large scale gradients in a    scale-down device cause fast transcriptional responses in    Corynebacterium glutamicum. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98, 8563–8572.
    2. 51. Buchholz,J., Graf,M., Blombach,B. and Takors,R. (2014) Improving the carbon balance of fermentations by total carbon analyses. BIOCHEMICAL ENGINEERING JOURNAL, 90, 162–169.
    3. 50. Gelves,R., Dietrich,A. and Takors,R. (2014) Modeling of gas-liquid mass transfer in a stirred tank bioreactor    agitated by a Rushton turbine or a new pitched blade impeller. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 37, 365–375.
    4. 49. Schuhmacher,T., Loeffler,M., Hurler,T. and Takors,R. (2014) Phosphate limited fed-batch processes: Impact on carbon usage and energy    metabolism in Escherichia coli. JOURNAL OF BIOTECHNOLOGY, 190, 96–104.
    5. 48. Lieder,S., Jahn,M., Seifert,J., von Bergen,M., Mueller,S. and Takors,R. (2014) Subpopulation-proteomics reveal growth rate, but not cell cycling, as a    major impact on protein composition in Pseudomonas putida KT2440. AMB EXPRESS, 4.
  8. 2013

    1. 47. Soellner,S., Rahnert,M., Siemann-Herzberg,M., Takors,R. and Altenbuchner,J. (2013) Evolution of pyruvate kinase-deficient Escherichia coli mutants enables    glycerol-based cell growth and succinate production. JOURNAL OF APPLIED MICROBIOLOGY, 115, 1368–1378.
    2. 46. Blombach,B., Buchholz,J., Busche,T., Kalinowski,J. and Takors,R. (2013) Impact of different CO2/HCO3- levels on metabolism and regulation in    Corynebacterium glutamicum. JOURNAL OF BIOTECHNOLOGY, 168, 331–340.
    3. 45. Buchholz,J., Schwentner,A., Brunnenkan,B., Gabris,C., Grimm,S., Gerstmeir,R., Takors,R., Eikmanns,B.J. and Blombach,B. (2013) Platform Engineering of Corynebacterium glutamicum with Reduced Pyruvate    Dehydrogenase Complex Activity for Improved Production of L-Lysine,    L-Valine, and 2-Ketoisovalerate. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79, 5566–5575.
    4. 44. Vallon,T., Glemser,M., Malca,S.H., Scheps,D., Schmid,J., Siemann-Herzberg,M., Hauer,B. and Takors,R. (2013) Production of 1-Octanol from n-Octane by Pseudomonas putida KT2440. CHEMIE INGENIEUR TECHNIK, 85, 841–848.
  9. 2012

    1. 43. Takors,R. (2012) Scale-up of microbial processes: Impacts, tools and open questions. JOURNAL OF BIOTECHNOLOGY, 160, 3–9.
  10. 2011

    1. 42. Bongaerts,J., Esser,S., Lorbach,V., Al-Momani,L., Mueller,M.A., Franke,D., Grondal,C., Kurutsch,A., Bujnicki,R., Takors,R., et al. (2011) Diversity-Oriented Production of Metabolites Derived from Chorismate and    Their Use in Organic Synthesis. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 50, 7781–7786.
    2. 41. Vielhauer,O., Zakhartsev,M., Horn,T., Takors,R. and Reuss,M. (2011) Simplified absolute metabolite quantification by gas    chromatography-isotope dilution mass spectrometry on the basis of    commercially available source material. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL  AND LIFE SCIENCES, 879, 3859–3870.
  11. 2009

    1. 40. Magnus,J.B., Oldiges,M. and Takors,R. (2009) The Identification of Enzyme Targets for the Optimization of a Valine    Producing Corynebacterium glutamicum Strain Using a Kinetic Model. BIOTECHNOLOGY PROGRESS, 25, 754–762.
  12. 2007

    1. 39. Noeh,K., Groenke,K., Luo,B., Takors,R., Oldiges,M. and Wiechert,W. (2007) Metabolic flux analysis at ultra short time scale: Isotopically    non-stationary C-13 labeling experiments. JOURNAL OF BIOTECHNOLOGY, 129, 249–267.
    2. 38. Luo,B., Groenke,K., Takors,R., Wandrey,C. and Oldiges,M. (2007) Simultaneous determination of multiple intracellular metabolites in    glycolysis, pentose phosphate pathway and tricarboxylic acid cycle by    liquid chromatography-mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1147, 153–164.
    3. 37. van der Werf,M.J., Takors,R., Smedsgaard,J., Nielsen,J., Ferenci,T., Portais,J.C., Wittmann,C., Hooks,M., Tomassini,A., Oldiges,M., et al. (2007) Standard reporting requirements for biological samples in metabolomics    experiments: microbial and in vitro biology experiments. METABOLOMICS, 3, 189–194.
    4. 36. Takors,R., Bathe,B., Rieping,M., Hans,S., Kelle,R. and Huthmacher,K. (2007) Systems biology for industrial strains and fermentation processes -    Example: Amino acids. JOURNAL OF BIOTECHNOLOGY, 129, 181–190.
  13. 2006

    1. 35. Wahl,S., Takors,R. and Wiechert,W. (2006) Interpretation of metabolic flux maps by limitation potentials and    constrained limitation sensitivities. BIOTECHNOLOGY AND BIOENGINEERING, 94, 263–272.
    2. 34. Magnus,J.B., Hollwedel,D., Oldiges,M. and Takors,R. (2006) Monitoring and modeling of the reaction dynamics in the valine/leucine    synthesis pathway in Corynebacterium glutamicum. BIOTECHNOLOGY PROGRESS, 22, 1071–1083.
  14. 2005

    1. 33. Ternbach,M., Bollman,C., Wandrey,C. and Takors,R. (2005) Application of model discriminating experimental design for modeling and    development of a fermentative fed-batch L-valine production process. BIOTECHNOLOGY AND BIOENGINEERING, 91, 356–368.
    2. 32. Oldiges,M. and Takors,R. (2005) Applying metabolic profiling techniques for stimulus-response    experiments: Chances and pitfalls. In TECHNOLOGY TRANSFER IN BIOTECHNOLOGY: FROM LAB TO INDUSTRY TO PRODUCTION, TECHNOLOGY TRANSFER IN BIOTECHNOLOGY: FROM LAB TO INDUSTRY TO PRODUCTION. SPRINGER-VERLAG BERLIN, HEIDELBERGER PLATZ 3, D-14197 BERLIN, GERMANY, Vol. 92, pp. 173–196.
    3. 31. Haunschild,M., Freisleben,B., Takors,R. and Wiechert,W. (2005) Investigating the dynamic behavior of biochemical networks using model    families. BIOINFORMATICS, 21, 1617–1625.
  15. 2004

    1. 30. Takors,R. (2004) Complete cell ISPR process development: Chances and risks. CHEMIE INGENIEUR TECHNIK, 76, 1857–1864.
    2. 29. Ruffer,N., Heidersdorf,U., Kretzers,I., Sprenger,G., Raeven,L. and Takors,R. (2004) Fully integrated L-phenylalanine separation and concentration using    reactive-extraction with liquid-liquid centrifuges in a fed-batch    process with E-coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 26, 239–248.
    3. 28. Takors,R. (2004) Model-based analysis and optimization of an ISPR approach using reactive    extraction for pilot-scale L-phenylalanine production. BIOTECHNOLOGY PROGRESS, 20, 57–64.
    4. 27. Zelic,B., Vasic-Racki,D., Wandrey,C. and Takors,R. (2004) Modeling of the pyruvate production with Escherichia coli in a fed-batch    bioreactor. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 26, 249–258.
    5. 26. Zelic,B., Gostovic,S., Vuorilehto,K., Vasic-Racki,B. and Takors,R. (2004) Process strategies to enhance pyruvate production with recombinant    Escherichia coli: from repetitive fed-batch to in situ product recovery    with fully integrated electrodialysis. BIOTECHNOLOGY AND BIOENGINEERING, 85, 638–646.
    6. 25. Degenring,D., Froemel,C., Dikta,G. and Takors,R. (2004) Sensitivity analysis for the reduction of complex metabolism models. JOURNAL OF PROCESS CONTROL, 14, 729–745.
    7. 24. Wahl,A., El Massaoudi,M., Schipper,D., Wiechert,W. and Takors,R. (2004) Serial C-13-based flux analysis of an L-phenylalanine-producing E-coli    strain using the sensor reactor. BIOTECHNOLOGY PROGRESS, 20, 706–714.
    8. 23. Drysch,A., El Massaoudi,M., Wiechert,W., de Graaf,A. and Takors,R. (2004) Serial flux mapping of Corynebacterium glutamicum during fed-batch    L-lysine production using the sensor reactor approach. BIOTECHNOLOGY AND BIOENGINEERING, 85, 497–505.
    9. 22. Oldiges,M., Kunze,M., Degenring,D., Sprenger,G. and Takors,R. (2004) Stimulation, monitoring, and analysis of pathway dynamics by metabolic    profiling in the aromatic amino acid pathway. BIOTECHNOLOGY PROGRESS, 20, 1623–1633.
    10. 21. Takors,R. (2004) Whole cell ISPR process development: Chances and pitfalls. CHEMIE INGENIEUR TECHNIK, 76, 1807–1814.
  16. 2003

    1. 20. Franke,D., Lorbach,V., Esser,S., Dose,C., Sprenger,G., Halfar,M., Thommes,J., Muller,R., Takors,R. and Muller,M. (2003) (S,S)-2,3-dihydroxy-2,3-dihydrobenzoic acid: Microbial access with    engineered cells of Escherichia coli and application as starting    material in natural-product synthesis. CHEMISTRY-A EUROPEAN JOURNAL, 9, 4188–4196.
    2. 19. Zelic,B., Gerharz,T., Bott,M., Vasic-Racki,D., Wandrey,C. and Takors,R. (2003) Fed-Batch Process for Pyruvate Production by Recombinant Escherichia    coli YYC202 Strain. ENGINEERING IN LIFE SCIENCES, 3, 299–305.
    3. 18. El Massaoudi,M., Spelthahn,J., Drysch,A., de Graaf,A. and Takors,R. (2003) Production process monitoring by serial mapping of microbial carbon flux    distributions using a novel sensor reactor approach: I - Sensor reactor    system. METABOLIC ENGINEERING, 5, 86–95.
    4. 17. Drysch,A., El Massaoudi,M., Mack,C., Takors,R., de Graaf,A. and Sahm,H. (2003) Production process monitoring by serial mapping of microbial carbon flux    distributions using a novel Sensor Reactor approach: II -    C-13-labeling-based metabolic flux analysis and L-lysine production. METABOLIC ENGINEERING, 5, 96–107.
  17. 2002

    1. 16. Gerigk,M., Maass,D., Kreutzer,A., Sprenger,G., Bongaerts,J., Wubbolts,M. and Takors,R. (2002) Enhanced pilot-scale fed-batch L-phenylalanine production with    recombinant Escherichia coli by fully integrated reactive extraction. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 25, 43–52.
    2. 15. Maass,D., Gerigk,M., Kreutzer,A., Weuster-Botz,D., Wubbolts,M. and Takors,R. (2002) Integrated L-phenylalanine separation in an E-coli fed-batch process:    from laboratory to pilot scale. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 25, 85–96.
    3. 14. Buchholz,A., Hurlebaus,J., Wandrey,C. and Takors,R. (2002) Metabolomics: Quantification of intracellular metabolite dynamics. Biomol. Eng., 19, 5–15.
    4. 13. Hurlebaus,J., Buchholz,A., Alt,W., Wiechert,W. and Takors,R. (2002) MMT – A Pathway Modeling Tool for Data From Rapid Sampling Experiments. In Silico Biology, 2, 467–484.
    5. 12. Gerigk,M., Bujnicki,R., Ganpo-Nkwenkwa,E., Bongaerts,J., Sprenger,G. and Takors,R. (2002) Process control for enhanced L-phenylalanine production using different    recombinant Escherichia coli strains. BIOTECHNOLOGY AND BIOENGINEERING, 80, 746–754.
    6. 11. Schmitz,M., Hirsch,E., Bongaerts,J. and Takors,R. (2002) Pulse experiments as a prerequisite for the quantification of in vivo    enzyme kinetics in aromatic amino acid pathway of Escherichia coli. BIOTECHNOLOGY PROGRESS, 18, 935–941.
  18. 2001

    1. 10. Curvers,S., Brixius,P., Klauser,T., Weuster-Botz,D., Takors,R. and Wandrey,C. (2001) Human chymotrypsinogen B production with Pichia pastoris by integrated fermentation and downstream processing Part I: Fermentation processing. Biotechnol. Prog, 17, 495–502.
    2. 9. Thömmes,J., Halfar,M., Gieren,H., Curvers,S., Takors,R., Brunschier,R. and Kula,M.R. (2001) Human chymotrypsinogen B production with Pichia pastoris by integrated fermentation and downstream processing Part II: Protein recovery. Biotechnol. Prog, 17, 503–512.
    3. 8. Takors,R., Gerigk,M., Paschold,H. and Wandrey,C. (2001) Principal-component analysis for microbial L-phenylalanine production. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 24, 93–99.
    4. 7. Buchholz,A., Takors,R. and Wandrey,C. (2001) Quantification of intracellular metabolites in Escherichia coli K12    using liquid chromatographic-electrospray ionization tandem mass    spectrometric techniques. ANALYTICAL BIOCHEMISTRY, 295, 129–137.
    5. 6. Curvers,S., Linneman,J., Klauser,T., Wandrey,C. and Takors,R. (2001) Recombinant protein production with Pichia pastoris in continuous    fermentation - Kinetic analysis of growth and product formation. CHEMIE INGENIEUR TECHNIK, 73, 1615–1621.
  19. 2000

    1. 5. Dilsen,S., Paul,W., Sandgathe,A., Tippe,D., Freudl,R., Thömmes,J., Kula,M.R., Takors,R., Wandrey,C. and Weuster-Botz,D. (2000) Fed-Batch Production of recombinant human calcitonin precursor fusion-protein using Staphylococcus carnosus as expression-sectretion system. Appl. Microbiol. Biotechnol., 54, 361–369.
    2. 4. Schmitz,G., Franke,D., Stevens,S., Takors,R., Weuster-Botz,D. and Wandrey,C. (2000) Regioselective oxidation of terfenadine with Cunninghamella blakesleeana. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 10, 313–324.
  20. 1999

    1. 3. Schaefer,U., Boos,W., Takors,R. and Weuster-Botz,D. (1999) Automated sampling device for monitoring intracellular metabolite    dynamics. ANALYTICAL BIOCHEMISTRY, 270, 88–96.
  21. 1997

    1. 2. Takors,R., Wiechert,W. and WeusterBotz,D. (1997) Experimental design for the identification of macrokinetic models and    model discrimination. BIOTECHNOLOGY AND BIOENGINEERING, 56, 564–576.

Member of educational programs at University of Stuttgart: Technical Biology, (Bio-) Chemical Engineering, Technical Kybernetics, Environmental Engineering:

  • Biochemical Engineering (6 ECTS, undergraduate course), summer and winter term
  • Metabolic engineering (3 ECTS, graduate course), winter term
  • Bioreaction engineering (3 ECTS graduate course), summer term
  • Downstream processing (3 ECTS graduate course), winter term
  • Multiple Practical Courses for undergraduates and graduates

Other Teaching activities:

  • 2014, 2015, 2017, 2018: Lecturer at TU Delft, Advanced course ‘Microbial Physiology and Fermentation’, Delft, The Netherland
  • 2016: Lecturer at TU Delft, Advanced Course ‘Genomics in Industrial Biotechnology, Delft, The Netherlands
  • 2016: Key closing lecture: European Summer School on Industrial Biotechnology, Barcelona, Spain
  • 2014: Lecturer German/Sino Graduate School, University of Hamburg-Harburg, Germany
  • 2012: Lecturer at Braunwald Course ‘Biosystems Engineering’, Braunwald Switzerland
  • 2011: Lecturer: Workshop ‘Synthetic Biology’, Basel, Switzerland
  • 2011: Lecturer: Scale-up and Technology Transfer, Sanofi-Aventis, Frankfurt, Germany

Since 01.07.2009

Director of the Institute of Biochemical Engineering (Institut für Bioverfahrenstechnik, IBVT), University of Stuttgart 


Evonik Degussa GmbH, business unit Health & Nutrition; responsible for bioprocess development from lab to production scale, systems metabolic engineering 


Professorial dissertation (Habilitation); Institut für Biotechnologie, Forschungszentrum Jülich GmbH / RWTH Aachen

Metabolic and Bioprocess Engineering – A Fruitful Symbiosis

Venia Legendi: Metabolic Engineering 


Dissertation Biochemical Engineering (Dr. degree), Institut für Biotechnologie, Forschungszentrum Jülich GmbH / RWTH Aachen 


Diploma: Mechanical and Process Engineering, RWTH Aachen, Germany 


Born in Koblenz, Germany, married, two children




Award: DECHEMA (Young-Professor Award) Hochschullehrer-Nachwuchspreis



  • since 2017: member of the editorial board microbial biotechnology
  • since 2013: member of the editorial advisory board Bioprocess and Biosystems Engineering
  • since 2012: member of the editorial board Engineering Life Science


  • since 10/2014: Head of the German Biochemical Engineering Section at DECHEMA e.V.  (
  • since 10/2009: member of the Board of the Stuttgart Centre of Systems Biology, University of Stuttgart
  • at University of Stuttgart: multifold member of recruitment panels and steady member of educational training committees
  • multifold memberships in external recruiting and evaluation panels


DECHEMA (Young-Professor Award) Hochschullehrer-Nachwuchspreis


Chmiel H., Takors R., Weuster-Botz D. 2018. Bioprozesstechnik. 4th edition (completely updated and newly structured) ISBN 978-3-8274-2476-1 (in press)


Pfizenmaier J., Takors, R. 2016. Host organisms: Mammalian Cells, In: 'Industrial Biotechnology', edited by James Liao and Christoph Wittmann. Wiley-VCH series edited by Sang Yup Lee, Jens Nielsen and Gregory Stephanopoulos (accepted)


Takors R. 2014. Kommentierte Formelsammlung Bioverfahrenstechnik, Springer Spektrum. ISBN 978-3-642-41903-4.


Takors R. 2013. Bioverfahrenstechnik. In: Industrielle Mikrobiologie (Eds. Sahm H, Antranikian G, Stahmann P, Takors R), 1st edition, Springer Spektrum. ISBN 978-3-8274-3039-7.


Takors R. 2010. Systembiologie in der Bioverfahrenstechnik. In: Bioprozesstechnik (Ed. Chmiel), 3rd edition, Springer Spektrum. ISBN 978-3-8274-2476-1.


Oldiges M, Takors* R. 2005. Applying Metabolic Profiling Techniques for Stimulus-Response Experiments: Chances and Pitfalls, Adv. Biochem. Eng./Biotechnol. 92:173-196.


Wiechert* W, Takors R. 2003. Validation of Metabolic Models: Concepts, Tools and Problems, 277 – 320, In: Metabolic Engineering in a Post Genomic Era (Eds.: H.V. Westerhoff, B.N. Kholodenko) Horizon Scientific Press, ISBN 0-9545232-2-9.


Haunschild* MD, Freisleben B, Wiechert W, Takors R. 2002. Distributed Simulation of Metabolic Networks. In: Modelling and Simulation 2002, 436-440. SCS Europe. ISBN: 90-77039-07-4., 16 th European Simulation Multiconference ESM’2002, Darmstadt, 03.-05.06. Conference Proceedings.


Degenring D, Takors R. 2002. In vivo investigation of metabolic dynamics in Escherichia coli K12 by evaluation of Rapid Sampling Experiments, Proceedings of 5 th German Workshop on Artificial Life, Lübeck, ISBN 3-89838-030-0, IOS Press.


Takors* R, Gerigk M, Maass D, Bongaerts J, Schmidt K, Sprenger G, Wubbolts M, Wandrey C. 2001. Fed-batch Fermentation with Integrated Reactive-Extraction for L-Phenylalanine Production, Proceedings of 8 th Int. Con. Comp. Appl. in Biotechnol. CAB8, 24.-27.06.2001, Quebec, Canada, pp 189-194, ISBN 0-08-043681-1.


Takors* R, Weuster-Botz D, Wiechert W, Wandrey C. 2001. A model discriminating approach for data analysis and experimental design. Focus on Biotechnology, Vol. IV:   111-128, Eds. M. Hofman and P. Thonart, ISBN 0-7923-6927-0.


Takors* R, Weuster-Botz D, Wiechert W, Wandrey C. 1998. Model discrimination and parameter identification by an experimental design strategy, IFAC Proceeding Volumes: Computer Applications in Biotechnology. Pergamon Press. ISBN 0-08-043051-1.


Michalowski, A., Siemann-Herzberg, M. Takors, R. 2017. Bacterial strain and method for high throughput of sugar in the microbial conversion into biosynthetic products. WO2017/186327 A1 


Takors, R., Tesch, M., Brandt, H.-J., Wandrey, C. 2004. Verfahren und Vorrichtung zur Begasung von Weinen, Deutsche Patentanmeldung DE 199 38 226.3 (August 1999), EP 1083221 granted 24.03.04. 


Bott, M., Gerharz, T., Zelic, B., Takors. R. 2002. Verfahren sowie Mikroorganismen zur mikrobiellen Herstellung von Pyruvat aus Kohlenhydraten sowie Alkoholen, DE 102 20 234.6, 06.05. 2002 


Rüffer, N., Wandrey, C., Takors, R. 2002. Integrierte Abtrennung organischer Substanzen aus einem wässrigen Bioprozessgemisch, DE 10220235.4-43 (06.05.02). 


Gerigk, M., Raeven, L., Sprenger, G., Takors, R. 2002. Process for the production of aromatic amino acid metabolites or derivatives thereof, (December 2001), EP 02078876.6 17.09.2002 


Gerharz, T., Bott, M., Zelic, B., Takors, R. 2001. Verfahren zur fermentativen Herstellung von Pyruvat, German patent application 101 29 711.4, 22.06. 2001 


El Massaoudi, M., Franz, A., de Graaf, A.A., Takors, R. 2001 Verfahren und Vorrichtung zur Untersuchung von Fermentationsprozessen, DE 101 20 839.1-41 (27.04.01) 


Müller M., Franke D., Müller, R., Thömmes, J.,Halfar, M., Sprenger, G., Takors, R., Lorbach, V. 2001. Herstellung von rekombinanten Mikroorganismen zur Produktion und Reingewinnung von Substanzen aus dem Aromaten-Biosyntheseweg und die Verwendung dieser in der Synthese biologisch wirksamer Substanzen, German patent application DE 100.42.535.6 (18.06.01) 


Buchholz, A., Takors, R., Wandrey, C. 2000. Verfahren zur Dosierung exakter Volumina von Flüssigkeiten in eine Vorlage sowie dafür geeignete Vorrichtung, Deutsche Patentanmeldung DE 100 03 416.0-52 (January 2000)


Gieren, H., Kula, M.-R., Rämsch, C., Selber, K., Thömmes, J., Halfar, M., Curvers, S., Takors, R., Weuster-Botz, D., Klauser, T. 1999. Verfahren zur Herstellung und Extraktion von Proteinen, German patent application DE 199 38 703.6-41 (August 1999)


Maaß, D., Takors, R., Paschold, H., Weuster-Botz, D., Wandrey, C. 2000. Verfahren zur Abtrennung organischer Substanzen aus einem wäßrigen Gemisch, German patent application DE 199 19 490.4-44 (April 1999, granted 18.04.2005), EP00/03705, WO 00/66253 (09.11.2000).


Schmitz, G., Weuster-Botz, D., Takors, R., Wandrey, C. 2002. Verfahren zur biokatalysierten Umsetzung schlecht wasserlöslicher Substanzen, German patent application 199 13 862.1-41 (März 1999), 05.11.2002.

  1. EmpowerPutida Project presentation, 2018 Movie
  2. Mit Fermentation das Klima retten, Deutschlandfunk, 2019 Radiointerview
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