Protein Synthesis Group

Robust Production Systems for Heterologous Protein Production.

Upstream Processing

Cell-Free Protein Synthesis

  • Identification of Systems Limiting Factors of Cell-Free Proteins Synthesis by Systems Biology Approaches 
  • In Vitro Production at preparative Scale
  • Reaction Performance: Microfluidic In Vitro Protein Assembly Line Based on Cell Free Reactor Systems
  • Synthetic Biology: Synthesis of Artificial Proteins, in Vitro and in Vivo.

In Vivo

  • Current Biological Systems: Escherichia coli, Bacillus subtilis, Pseudomonas putida, Vibrio, Corynebacteria, Yeasts & Mammalian Cell Cultures
  • Robust Technical Expression Based on Global Regulator Systems
  • Process Development of High Cell Density Cultivations (hcd)
  • Periplasmatic Protein Production (E. coli)
  • Robust Algorithm for Predicting Target-Dependent Gene Expression
  • Therapeutic Protein Production in Mammalian Cell Cultures

Downstream Processing

  • Immobilized Metal Affinity Ligand Technology (Magnetic separation)
  • Integrative Process Development

Selected Publications

  1. Niess,A., Siemann-Herzberg,M. and Takors,R. (2019) Protein Production in Escherichia Coli Is Guided by the Trade-off between Intracellular Substrate Availability and Energy Cost. Microbial Cell Factory, 18.
  2. Failmezger,J., Scholz,S., Blombach,B. and Siemann-Herzberg,M. (2018) Cell-Free Protein Synthesis From Fast-Growing Vibrio Natriegens. Frontiers in Microbiology, 9.
  3. Failmezger,J., Ludwig,J., Niess,A. and Siemann-Herzberg,M. (2017) Quantifying Ribosome Dynamics in Escherichia Coli Using Fluorescence. FEMS MICROBIOLOGY LETTERS, 364.
  4. Niess,A., Failmezger,J., Kuschel,M., Siemann-Herzberg,M. and Takors,R. (2017) Experimentally Validated Model Enables Debottlenecking of in Vitro  Protein Synthesis and Identifies a Control Shift under in Vivo  Conditions. ACS SYNTHETIC BIOLOGY, 6, 1913–1921.
  5. Failmezger,J., Rauter,M., Nitschel,R., Kraml,M. and Siemann-Herzberg,M. (2017) Cell-Free Protein Synthesis from Non-Growing, Stressed Escherichia Coli. SCIENTIFIC REPORTS, 7.
  6. Failmezger,J., Nitschel,R., Sanchez-Kopper,A., Kraml,M. and Siemann-Herzberg,M. (2016) Site-Specific Cleavage of Ribosomal RNA in Escherichia Coli-Based  Cell-Free Protein Synthesis Systems. PLoS One, 11.
  7. Oelschlaeger,P., Lange,S., Schmitt,J., Siemann,M., Reuss,M. and Schmid,R. (2003) Identification of Factors Impeding the Production of a Single-Chain  Antibody Fragment in Escherichia Coli by Comparing in Vivo and in Vitro  Expression. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 61, 123–132.
  8. Arnold,S., Siemann,M., Scharnweber,K., Werner,M., Baumann,S. and Reuss,M. (2001) Kinetic Modeling and Simulation of in Vitro Transcription by Phage T7  RNA Polymerase. BIOTECHNOLOGY AND BIOENGINEERING, 72, 548–561.
  9. Schindler,P., Baumann,S., Reuss,M. and Siemann,M. (2000) In Vitro Coupled Transcription Translation: Effects of Modification in    Lysate Preparation on Protein Composition and Biosynthesis Activity. ELECTROPHORESIS, 21, 2606–2609.
  10. Schindler,P., Macherhammer,F., Arnold,S., Reuss,M. and Siemann,M. (1999) Investigation of Translation Dynamics under Cell-Free Protein  Biosynthesis Conditions Using High-Resolution Two-Dimensional Gel  Electrophoresis. ELECTROPHORESIS, 20, 806–812.
  11. Katanaev,V., Spirin,A., Reuss,M. and Siemann,M. (1996) Formation of Bacteriophage MS2 Infectious Units in a Cell-Free  Translation System. FEBS LETTERS, 397, 143–148.

For details please contact

Martin Siemann-Herzberg
apl. Prof. Dr. habil.

Martin Siemann-Herzberg

apl. Professor/ Vice Director

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