Protein Synthesis Group

Robust Production Systems for Heterologous Protein Production.

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Upstream Processing

Cell-Free Protein Synthesis

  • Identification of Systems Limiting Factors of Cell-Free Proteins Synthesis by Systems Biology Approaches 
  • In Vitro Production at preparative Scale
  • Reaction Performance: Microfluidic In Vitro Protein Assembly Line Based on Cell Free Reactor Systems
  • Synthetic Biology: Synthesis of Artificial Proteins, in Vitro and in Vivo.

In Vivo

  • Current Biological Systems: Escherichia coli, Bacillus subtilis, Pseudomonas putida, Vibrio, Corynebacteria, Yeasts & Mammalian Cell Cultures
  • Robust Technical Expression Based on Global Regulator Systems
  • Process Development of High Cell Density Cultivations (hcd)
  • Periplasmatic Protein Production (E. coli)
  • Robust Algorithm for Predicting Target-Dependent Gene Expression
  • Therapeutic Protein Production in Mammalian Cell Cultures

Downstream Processing

  • Immobilized Metal Affinity Ligand Technology (Magnetic separation)
  • Integrative Process Development

Selected Publications

  1. . Niess,A., Siemann-Herzberg,M. and Takors,R. (2019) Protein Production in Escherichia Coli Is Guided by the Trade-off between Intracellular Substrate Availability and Energy Cost. Microbial Cell Factory, 18.
  2. . Failmezger,J., Scholz,S., Blombach,B. and Siemann-Herzberg,M. (2018) Cell-Free Protein Synthesis from Fast-Growing Vibrio Natriegens. Frontiers in Microbiology, 9.
  3. . Failmezger,J., Rauter,M., Nitschel,R., Kraml,M. and Siemann-Herzberg,M. Cell-free protein synthesis from non-growing, stressed Escherichia coli. SCIENTIFIC REPORTS, 7.
  4. . Niess,A., Failmezger,J., Kuschel,M., Siemann-Herzberg,M. and Takors,R. Experimentally Validated Model Enables Debottlenecking of in Vitro Protein Synthesis and Identifies a Control Shift under in Vivo Conditions. ACS SYNTHETIC BIOLOGY, 6, 1913–1921.
  5. . Failmezger,J., Ludwig,J., Niess,A. and Siemann-Herzberg,M. Quantifying ribosome dynamics in Escherichia coli using fluorescence. FEMS MICROBIOLOGY LETTERS, 364.
  6. . Failmezger,J., Nitschel,R., Sanchez-Kopper,A., Kraml,M. and Siemann-Herzberg,M. Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems. PLoS One, 11.
  7. . Oelschlaeger,P., Lange,S., Schmitt,J., Siemann,M., Reuss,M. and Schmid,R. Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 61, 123–132.
  8. . Arnold,S., Siemann,M., Scharnweber,K., Werner,M., Baumann,S. and Reuss,M. Kinetic modeling and simulation of in vitro transcription by phage T7 RNA polymerase. BIOTECHNOLOGY AND BIOENGINEERING, 72, 548–561.
  9. . Schindler,P., Baumann,S., Reuss,M. and Siemann,M. In vitro coupled transcription translation: Effects of modification in lysate preparation on protein composition and biosynthesis activity. ELECTROPHORESIS, 21, 2606–2609.
  10. . Schindler,P., Macherhammer,F., Arnold,S., Reuss,M. and Siemann,M. Investigation of translation dynamics under cell-free protein biosynthesis conditions using high-resolution two-dimensional gel electrophoresis. ELECTROPHORESIS, 20, 806–812.
  11. . Katanaev,V., Spirin,A., Reuss,M. and Siemann,M. Formation of bacteriophage MS2 infectious units in a cell-free translation system. FEBS LETTERS, 397, 143–148.

For details please contact

This image shows Martin Siemann-Herzberg

Martin Siemann-Herzberg

apl. Prof. Dr. habil.

apl. Professor/ Vice Director

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